First order, Area under curve and dual wavelength Spectrophotometric methods has been developed for simultaneous estimation of Montelukast sodium and Acebrophylline in bulk drug and in pharmaceutical formulation. All these methods utilizes methanol as a solvent. In First order spectroscopy 236.4 nm and 334.4 nm were selected as ZCP (zero crossing point) for determination of both the drugs simultaneously. In Area Under Curve the wavelength ranges of 277-287 nm and 270-280 nm were selected as analytical wavelength ranges for the determination of area of both the drugs in pure and in combined formulation. In Dual wavelength method, the absorbance difference was determined within 244 nm and 254 nm for the determination of Montelukast sodium, and absorbance difference within 265 nm and 285 nm for the determination of Acebrophylline. The results of analysis of all methods have been validated statistically. Lower value of % RSD indicates that these methods were simple, rapid and accurate, hence can be used for routine simultaneous estimation of these drugs in formulations. The results were found to be within acceptance criteria according to ICH Q2 R1 guideline.
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